RNA

Part:BBa_K1942001

Designed by: Yingzi Chai   Group: iGEM16_NJU-China   (2016-10-10)


This part is a short RNA sequence designed for KRAS gene silencing. It is used for down-regulating K

This part is a short hairpin RNA (shRNA) sequence. When this sequence is cut by restriction enzyme and then integrated into pcDNA 6.2 vector, it can play an RNAi function in mammalian cell lines such as A549 cells. When the shRNA vector loading anti-KRAS is transfected into A549 cells, the shRNA hairpin structure will be cleaved into anti-KRAS siRNA by Dicer and then construct the RNA-induced silencing complex (RISC). The siRNA-RISC complex will target at KRAS mRNA under the guide of siRNA sequence and digest it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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